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efluor 450 cell proliferation dye (cpd, ebioscience)  (Thermo Fisher)


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    Thermo Fisher efluor 450 cell proliferation dye (cpd, ebioscience)
    Efluor 450 Cell Proliferation Dye (Cpd, Ebioscience), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+proliferation+dye+(cpd)+efluor+450/cell+proliferation+dye+efluor+670/pm33633313-52-13-19
    Average 90 stars, based on 1 article reviews
    efluor 450 cell proliferation dye (cpd, ebioscience) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Intravenous administration of viral vectors expressing prostate cancer antigens enhances the magnitude and functionality of CD8+ T cell responses.
    Article Snippet: Equal volumes of 0.4 μM or 4 μM Cell Proliferation Dye (CPD) eFluor 450 (eBioscience) were added to the mock- pulsed and PSA nonamer- pulsed cell populations, respectively, labeling cells with final concentrations of 0.2 μM or 2 μM CPD.

    Article Title: Liver type 1 innate lymphoid cells lacking IL-7 receptor are a native killer cell subset fostered by parenchymal niches
    Article Snippet: To assess the stability and the proliferation capacity of 7 R− ILC1s and 7R+ ILC1s in inflammatory conditions, CD45.2 WT mice were injected with indicated G1- ILC populations labeled by Cell Proliferation Dye (CPD) eFluor 450 (Thermo Fisher Scientific) (day 0) followed by the administration of 2 μg IL- 15/IL- 15Rα at days 1, 3, and 5.

    Article Title: Intravenous administration of viral vectors expressing prostate cancer antigens enhances the magnitude and functionality of CD8+ T cell responses
    Article Snippet: Equal volumes of 0.4 μM or 4 μM Cell Proliferation Dye (CPD) eFluor 450 (eBioscience) were added to the mock-pulsed and PSA nonamer-pulsed cell populations, respectively, labeling cells with final concentrations of 0.2 μM or 2 μM CPD.

    Article Title: Interleukin-10 counteracts T-helper type 1 responses in B-cell lymphoma and is a target for tumor immunotherapy.
    Article Snippet: To establish strategies for immunotherapy of B-cell lymphoma, it is mandatory to gain deeper insights into the mechanisms of tumor immune escape.. In a mouse model of endogenously arising lymphoma, we investigated the impact of IL-10 on the regulation of antitumor responses.. Despite progressive functional impairment of NK cells and lack of IFN-γ in the tumor milieu, we found an augmented fraction of T helper type 1 (Th1) cells, which continued to express IFN-γ but also upregulated IL-10 during disease development.

    Article Title: Efficient isolation of human gingival stem cells in a new serum-free medium supplemented with platelet lysate and growth hormone for osteogenic differentiation enhancement
    Article Snippet: Briefly, GSCs were seeded in a 48-well plate (50,000 cell/plate) in NA + G and A + G media and put in a humid 37 °C incubator with 5% CO 2 for 1 h. Meanwhile, PBMCs were treated with Cell Proliferation Dye (CPD) eFluor 450 (Cat. No. 65-0842-85, eBioscience) diluted (1:1000) in a PBS/FCS 3% solution for 10 min at 37 °C in the dark.

    Article Title: Liver type 1 innate lymphoid cells lacking IL-7 receptor are a native killer cell subset fostered by parenchymal niches
    Article Snippet: To assess the stability and the proliferation capacity of 7 R − ILC1s and 7R + ILC1s in inflammatory conditions, CD45.2 WT mice were injected with indicated G1-ILC populations labeled by Cell Proliferation Dye (CPD) eFluor 450 (Thermo Fisher Scientific) (day 0) followed by the administration of 2 μg IL-15/IL-15Rα at days 1, 3, and 5.

    Article Title: Immunostimulatory Potential of Extracellular Vesicles Isolated from an Edible Plant, Petasites japonicus , via the Induction of Murine Dendritic Cell Maturation
    Article Snippet: The maturation, activation, and phenotypic alteration of DCs and T cells were analyzed by flow cytometry (FACSverse, BD Bioscience, Bergen County, NJ, USA) using the following fluorescence-conjugated Abs: Live/Dead Cell Staining Kit (L/D; BV510) from Invitrogen (Carlsbad, CA, USA); Cell Proliferation Dye (CPD) eFluor 450, anti-MHC-I (APC), -MHC-II (PE-Cy7), -CD3 (APC-Cy7), -CD4 (Alexa488), -CD8α (Percp-Cy5.5), -IFN-γ (PE), -TNF-α (APC), and -IL-2 (PE-Cy7) Abs from eBioscience (San Diego, CA, USA); anti-CD11c (PE-Cy7), -CD80 (FITC), -CD86 (PE), -TNF-α (APC), -IL-12p70 (PE), and -IL-10 (FITC) Abs from BD Bioscience.

    Cytotoxicity Assay:

    Article Title: Siglec-6 is a target for chimeric antigen receptor T-cell treatment of chronic lymphocytic leukemia.
    Article Snippet: Stained cells were acquired on an LSRII instrument (BD) or NovoCyte (ACEA Bioscience, San Diego, CA) and analyzed using FlowJo software (Tree Star, Ashland, OR). .. Cell proliferation and cytotoxicity assay CAR-transduced T cells were labeled with 10 μM eFluor 450 cell proliferation dye (CPD, eBioscience) according to the manufacturer’s instructions. .. 5 × 105 CPD-labeled transduced CAR T cells were plated on 24 well/plates coated with recombinant human Siglec-6/Fc chimera protein (R&D System, Minneapolis, MN).

    Labeling:

    Article Title: Siglec-6 is a target for chimeric antigen receptor T-cell treatment of chronic lymphocytic leukemia.
    Article Snippet: Stained cells were acquired on an LSRII instrument (BD) or NovoCyte (ACEA Bioscience, San Diego, CA) and analyzed using FlowJo software (Tree Star, Ashland, OR). .. Cell proliferation and cytotoxicity assay CAR-transduced T cells were labeled with 10 μM eFluor 450 cell proliferation dye (CPD, eBioscience) according to the manufacturer’s instructions. .. 5 × 105 CPD-labeled transduced CAR T cells were plated on 24 well/plates coated with recombinant human Siglec-6/Fc chimera protein (R&D System, Minneapolis, MN).



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